Reversible phosphorylation of 3-hydroxy-3-methylglutaryl CoA reductase in morris hepatomas

Gregg R.G. and Wilce P.A. (1983) Reversible phosphorylation of 3-hydroxy-3-methylglutaryl CoA reductase in morris hepatomas. Biochemical and Biophysical Research Communications, 114 2: 473-478. doi:10.1016/0006-291X(83)90804-5


Author Gregg R.G.
Wilce P.A.
Title Reversible phosphorylation of 3-hydroxy-3-methylglutaryl CoA reductase in morris hepatomas
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1983-07-29
Sub-type Article (original research)
DOI 10.1016/0006-291X(83)90804-5
Volume 114
Issue 2
Start page 473
End page 478
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The reversible phosphorylation of microsomal 3-hydroxy-3-methylglutaryl CoA reductase in host liver and hepatoma 5123C has been investigated. The percentage of the total enzyme activity in vivo was similar in the normal liver, host liver and hepatoma 5123C. The inclusion of 30 mM EDTA and 10 mM mevalonic acid in assays of 3-hydroxy-3-methyl-glutaryl CoA reductase inactivation in vitro eliminated artifacts generated by the presence of mevalonate kinase. Inactivation of 3-hydroxy-3-methyl glutaryl CoA reductase from normal liver, host liver and hepatoma occurred at a similar rate with similar half-times. We conclude that phosphorylation/dephosphorylation of 3-hydroxy-3-methylglutaryl CoA reductase occurs in hepatomas and that the lack of dietary cholesterol feedback inhibition in the hepatomas is not a result of a defect in this particular aspect of the reversible phosphorylation system.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 26 Jul 2016, 03:53:26 EST by System User