On the latency, multiplicity, and subcellular distribution of catalase activity in mammalian tissues

Holmes R.S. and Masters C.J. (1970) On the latency, multiplicity, and subcellular distribution of catalase activity in mammalian tissues. International Journal of Biochemistry, 1 4: 474-482. doi:10.1016/0020-711X(70)90062-5


Author Holmes R.S.
Masters C.J.
Title On the latency, multiplicity, and subcellular distribution of catalase activity in mammalian tissues
Journal name International Journal of Biochemistry   Check publisher's open access policy
ISSN 0020-711X
Publication date 1970
Sub-type Article (original research)
DOI 10.1016/0020-711X(70)90062-5
Volume 1
Issue 4
Start page 474
End page 482
Total pages 9
Subject 1303 Specialist Studies in Education
Abstract 1. 1. The latency, multiplicity, and subcellular distribution of catalase activity have been studied in a variety of mammalian tissues, in order to clarify comparative aspects of the localization and isoenzyme status of this enzyme. 2. 2. Much of the catalase activity was found to be of a soluble nature, but the proportion of soluble contribution to the total catalase activity varied between species and between different tissues. Sheep liver displayed an especially high proportion of activity in the soluble form. 3. 3. The comparative efficiency of a number of solubilization procedures was studied, and blending with 0.05 per cent Triton X-100 was demonstrated as the most efficient means of releasing paniculate catalase activity. 4. 4. Representative enzymes, resulting from this solubilization procedure, were resolved into multiple forms by high-voltage starch-gel electrophoresis. In this way, evidence was provided for a complex heterogeneity of the enzyme, and for a differential distribution of the multiple enzyme forms.
Keyword Catalase
differential distribution
enzyme heterogeneity
kidney
latency
mammalian liver
mice
multiplicity
rats
sheep
subcellular distribution
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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