Effects of albumin sensitization and challenge of guinea pigs on uptake2 of isoprenaline in trachealis muscle

Bryan-Lluka L.J. and Vuocolo H.E. (1994) Effects of albumin sensitization and challenge of guinea pigs on uptake2 of isoprenaline in trachealis muscle. Naunyn-Schmiedeberg's Archives of Pharmacology, 349 6: 578-582. doi:10.1007/BF01258462


Author Bryan-Lluka L.J.
Vuocolo H.E.
Title Effects of albumin sensitization and challenge of guinea pigs on uptake2 of isoprenaline in trachealis muscle
Journal name Naunyn-Schmiedeberg's Archives of Pharmacology   Check publisher's open access policy
ISSN 0028-1298
Publication date 1994
Sub-type Article (original research)
DOI 10.1007/BF01258462
Volume 349
Issue 6
Start page 578
End page 582
Total pages 5
Publisher Springer-Verlag
Subject 3004 Pharmacology
Abstract There is evidence that hyperpolarization of cells by various mechanisms results in stimulation of uptake2 of catecholamines and, conversely, that depolarization causes inhibition of uptake2. The aim of this study was to examine whether changes that have been shown to occur in the resting membrane potential of the trachealis muscle of guinea pigs that have been sensitized to albumin (hyperpolarization) or albumin-sensitized followed by inhalational albumin challenge (depolarization) are reflected in changes in uptake2 in the smooth muscle. Uptake2 of isoprenaline (as measured by the steady-state rate of specific3H-O-methylisoprenaline formation normalized for the isoprenaline concentration) was determined in isolated segments of trachealis muscle that were incubated in 3H-(±)-isoprenaline and were from guinea pigs from three treatment groups: (i) controls, (ii) albumin-sensitized and (iii) albumin-sensitized and challenged. At an isoprenaline concentration that does not hyperpolarize the trachealis muscle (1 nmol/l), uptake2 was significantly greater in the muscle from sensitized guinea pigs than that from control or sensitized and challenged guinea pigs. When a drug that hyperpolarized the trachealis muscle was present (25 nmol/l isoprenaline or 10 μmol/l (-)-cromakalim), there were no differences in uptake2 between the three groups. Propranolol prevented the stimulation of uptake2 by isoprenaline and glibenclamide prevented stimulation of uptake2 by (-)-cromakalim. In the presence of propranolol or glibenclamide, there were no differences in uptake2 between the three treatment groups of guinea pigs. It is concluded from these results that uptake2 of isoprenaline in the trachealis muscle appears to reflect the resting membrane potential in albumin-sensitized guinea pigs, whether they were also albumin challenged or not, but these differences are not maintained in the presence of drugs that change the membrane potential.
Keyword Albumin sensitization and challenge
Guinea-pig trachealis
Isoprenaline
Membrane potential
Uptake2
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 26 Jul 2016, 02:25:16 EST by System User