Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein

Bartley, Paul A., Keough, Rebecca A., Lutwyche, Jodie K. and Gonda, Thomas J. (2003) Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein. Oncogene, 22 48: 7570-7575. doi:10.1038/sj.onc.1207136


Author Bartley, Paul A.
Keough, Rebecca A.
Lutwyche, Jodie K.
Gonda, Thomas J.
Title Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein
Journal name Oncogene   Check publisher's open access policy
ISSN 0950-9232
1476-5594
Publication date 2003
Sub-type Article (original research)
DOI 10.1038/sj.onc.1207136
Volume 22
Issue 48
Start page 7570
End page 7575
Total pages 6
Place of publication London, United Kingdom
Publisher Nature
Language eng
Abstract The identification of Myb 'target' genes will not only aid in the understanding of how overexpression of Myb, or expression of activated forms of Myb, leads to cellular transformation but will also shed light on its role in normal cells. Using a combination of an estrogen-regulated Myb-transformed cell line (ERMYB) and PCR-based subtractive hybridization, we have identified the gene (GSTM1) encoding the detoxification enzyme glutathione S-transferase M1 as being transcriptionally upregulated by Myb. Functional analysis of the GSTM1 promoter using reporter assays indicated that both the DNA binding and transactivation domains of Myb were required for transcriptional activation. Mutational analysis of consensus Myb-binding sites (MBS) in the promoter and electrophoretic mobility gel shift analysis indicated that one of the three potential MBS can bind Myb protein, and is the primary site involved in the regulation of this promoter by Myb.
Keyword Glutathione S-transferase
Myb
Promoter
Transactivation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Mon, 13 Aug 2007, 13:53:27 EST