Impact of ERK activation on fly survival and Wolbachia-mediated protection during virus infection

Wong, Zhee Sheen, Brownlie, Jeremy C. and Johnson, Karyn N. (2016) Impact of ERK activation on fly survival and Wolbachia-mediated protection during virus infection. Journal of General Virology, 97 6: 1446-1452. doi:10.1099/jgv.0.000456


Author Wong, Zhee Sheen
Brownlie, Jeremy C.
Johnson, Karyn N.
Title Impact of ERK activation on fly survival and Wolbachia-mediated protection during virus infection
Formatted title
Impact of ERK activation on fly survival and Wolbachia-mediated protection during virus infection
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 1465-2099
0022-1317
Publication date 2016-06-01
Year available 2016
Sub-type Article (original research)
DOI 10.1099/jgv.0.000456
Open Access Status Not Open Access
Volume 97
Issue 6
Start page 1446
End page 1452
Total pages 7
Place of publication London, United Kingdom
Publisher Microbiology Society
Collection year 2017
Language eng
Formatted abstract
Elevated levels of reactive oxygen species (ROS) provide protection against virus-induced mortality in Drosophila. In addition to contributing to oxidative stress, ROS are known to activate a number of signalling pathways including the extracellular signal-regulated kinases (ERK) signalling cascade. It was recently shown that ERK signalling is important for resistance against viral replication and invasion in cultured Drosophila cells and the gut epithelium of adult flies. Here, using a Drosophila loss-of-function ERK (rolled) mutant we demonstrated that ERK is important for fly survival during virus infection. ERK mutant flies subjected to Drosophila C virus (DCV) oral and systemic infection were more susceptible to virus-induced mortality as compared with wild-type flies. We have demonstrated experimentally that ERK activation is important for fly survival during oral and systemic virus infection. Given that elevated ROS correlates with Wolbachia-mediated antiviral protection, we also investigated the involvement of ERK in antiviral protection in flies infected by Wolbachia. The results indicate that ERK activation is increased in the presence of Wolbachia but this does not appear to influence Wolbachia-mediated antiviral protection, at least during systemic infection.
Keyword Reactive oxygen species (ROS)
Drosophila
Extracellular signal-regulated kinases (ERK)
Wolbachia
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Sun, 24 Jul 2016, 00:22:54 EST by System User on behalf of Learning and Research Services (UQ Library)