Probiotics modify tight-junction proteins in an animal model of nonalcoholic fatty liver disease

Briskey, David, Heritage, Mandy, Jaskowski, Lesley-Anne, Peake, Jonathan, Gobe, Glenda, Subramaniam, V. Nathan, Crawford, Darrell, Campbell, Catherine and Vitetta, Luis (2016) Probiotics modify tight-junction proteins in an animal model of nonalcoholic fatty liver disease. Therapeutic Advances in Gastroenterology, 9 4: 463-472. doi:10.1177/1756283X16645055


Author Briskey, David
Heritage, Mandy
Jaskowski, Lesley-Anne
Peake, Jonathan
Gobe, Glenda
Subramaniam, V. Nathan
Crawford, Darrell
Campbell, Catherine
Vitetta, Luis
Title Probiotics modify tight-junction proteins in an animal model of nonalcoholic fatty liver disease
Journal name Therapeutic Advances in Gastroenterology   Check publisher's open access policy
ISSN 1756-2848
1756-283X
Publication date 2016-07-01
Year available 2016
Sub-type Article (original research)
DOI 10.1177/1756283X16645055
Open Access Status Not Open Access
Volume 9
Issue 4
Start page 463
End page 472
Total pages 10
Place of publication London, United Kingdom
Publisher Sage Publications
Language eng
Formatted abstract
Background: We have investigated the effects of a multispecies probiotic preparation containing a combination of probiotic bacterial genera that included Bifidobacteria, Lactobacilli and a Streptococcus in a mouse model of high-fat diet or obesity-induced liver steatosis.

Methods: Three groups of C57B1/6J mice were fed either a standard chow or a high-fat diet for 20 weeks, while a third group was fed a high-fat diet for 10 weeks and then concomitantly administered probiotics for a further 10 weeks. Serum, liver and large bowel samples were collected for analysis.

Results: The expression of the tight-junction proteins ZO-1 and ZO-2 was reduced (p < 0.05) in high-fat diet-fed mice compared to chow-fed mice. Probiotic supplementation helped to maintain tight ZO-1 and ZO-2 expression compared with the high-fat diet group (p < 0.05), but did not restore ZO-1 or ZO-2 expression compared with chow-fed mice. Mice fed a high-fat diet ± probiotics had significant steatosis development compared with chow-fed mice (p < 0.05); steatosis was less severe in the probiotics group compared with the high-fat diet group. Hepatic triglyceride concentration was higher in mice fed a high-fat diet ± probiotics compared with the chow group (p < 0.05), and was lower in the probiotics group compared with the high-fat diet group (p < 0.05). Compared with chow-fed mice, serum glucose, cholesterol concentration and the activity of alanine transaminase were higher (p < 0.05), whereas serum triglyceride concentration was lower (p < 0.05) in mice fed a high-fat diet ± probiotics.

Conclusions: Supplementation with a multispecies probiotic formulation helped to maintain tight-junction proteins ZO-1 and ZO-2, and reduced hepatic triglyceride concentration compared with a high-fat diet alone.
Keyword Dysbiosis
Nonalcoholic fatty liver disease
Probiotics
Steatosis
Tight-junction proteins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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