Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model

Johnston, Stephen, Lopez-Fernandez, Carmen, Arroyo, Francisca, Gosalbez, Altea, Cortes Gutierrez, Elva I., Fernandez, Jose-Luis and Gosalvez, Jaime (2016) Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model. Journal of Assisted Reproduction and Genetics, . doi:10.1007/s10815-016-0754-9


Author Johnston, Stephen
Lopez-Fernandez, Carmen
Arroyo, Francisca
Gosalbez, Altea
Cortes Gutierrez, Elva I.
Fernandez, Jose-Luis
Gosalvez, Jaime
Title Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model
Journal name Journal of Assisted Reproduction and Genetics   Check publisher's open access policy
ISSN 1058-0468
1573-7330
Publication date 2016-07-02
Sub-type Article (original research)
DOI 10.1007/s10815-016-0754-9
Open Access Status Not Open Access
Total pages 8
Place of publication New York, United States
Publisher Springer
Collection year 2017
Language eng
Formatted abstract
Purpose: Using a rabbit model, we assessed the influence of sperm DNA longevity on female reproductive outcomes.

Methods: Semen was collected from 40 bucks, incubated at 38 °C for 24 h, and the rate of sperm DNA fragmentation (rSDF) was determined using the sperm chromatin dispersion assay. Males were allocated into high rSDF (>0.5 units of increase per hour) or low rSDF (<0.5 units of increase per hour) groups. High and low rSDF semen samples were sequentially artificially inseminated into the same doe to reduce female factor variability, and pregnancy outcomes were recorded.

Results: While there was no difference in SDFs between rSDF groups immediately after collection (T0), differences were significant after 2 h of incubation; SDFs determined at collection and rSDF behaved as independent characters (Pearson correlation = 0.099; P = 0.542). Following artificial insemination, the rate of stillborn pups was significantly higher in does inseminated by males with a high rSDF (14/21) compared to those with low rSDF (15/6); (contingency χ2 5.19; p = 0.022). The risk of stillborn when low rSDF rabbits were used for insemination was 0.16, but increased to 0.36 when high rSDF animals were used (odds ratio = 2.85; 95 % confidence interval = 1.4–2.7).

Conclusion(s): Dynamic assessment of SDF coupled with natural multiple ovulation, high fecundity of the rabbit and control over female factor influence, provided a useful experimental model to demonstrate the adverse effect of reduced sperm DNA longevity on reproductive outcome.
Keyword Oryctolagus cuniculus
Sperm DNA fragmentation
Still born
Sperm DNA longevity
Dynamic assay
Animal model
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Thu, 21 Jul 2016, 19:23:58 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences