Involvement of CRH-R2 receptor in eating behavior and in the response of the HPT axis in rats subjected to dehydration-induced anorexia

de Gortari, Patricia, Mancera, Karen, Cote-Velez, Antonieta, Isabel Amaya, Maria, Martinez, Adrian, Jaimes-Hoy, Lorraine and Joseph-Bravo, Patricia (2009) Involvement of CRH-R2 receptor in eating behavior and in the response of the HPT axis in rats subjected to dehydration-induced anorexia. Psychoneuroendocrinology, 34 2: 259-272. doi:10.1016/j.psyneuen.2008.09.010


Author de Gortari, Patricia
Mancera, Karen
Cote-Velez, Antonieta
Isabel Amaya, Maria
Martinez, Adrian
Jaimes-Hoy, Lorraine
Joseph-Bravo, Patricia
Title Involvement of CRH-R2 receptor in eating behavior and in the response of the HPT axis in rats subjected to dehydration-induced anorexia
Journal name Psychoneuroendocrinology   Check publisher's open access policy
ISSN 0306-4530
1873-3360
Publication date 2009-02
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.psyneuen.2008.09.010
Open Access Status Not Open Access
Volume 34
Issue 2
Start page 259
End page 272
Total pages 14
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Formatted abstract
Wistar rats subjected to dehydration-induced anorexia (DIA), with 2.5% NaCl solution as drinking water for 7 days, decrease by 80% their food intake and present some changes common to pair-fed food restricted rats (FFR) such as: weight loss, decreased serum leptin and expression of orexigenic arcuate peptides, increasing the anorexigenic ones and serum corticosterone levels. In contrast, the response of the HPT axis differs: DIA animals have increased TRH expression in PVN and present primary as opposed to the tertiary hypothyroidism of the FFR. Exclusive to DIA is the activation of CRHergic neurons in the lateral hypothalamus (LH) that project to PVN. Since TRH neurons of the PVN contain CRH receptors, we hypothesized that the differences in the response of the HPT axis to DIA could be due to CRH regulating TRHergic neurons. CRH effect was first evaluated on TRH expression of cultured hypothalamic cells where TRH mRNA levels increased after 1 h with 0.1 nM of CRH. We then measured the mRNA levels of CRH receptors in the PVN of male and female rats subjected to DIA; only those of CRH-R2 were modulated (down-regulated). The CRH-R2 antagonist antisauvagine-30 was therefore injected into the PVN of male rats, during the 7 days of DIA. Antisauvagine-30 induced a higher food intake than controls, and impeded the changes produced by DIA on the HPT axis: PVN TRH mRNA, and serum TH and TSH levels were decreased to similar values of FFR animals. Results corroborate the anorexigenic effect of CRH and show its role, acting through CRH-R2 receptors, in the activation of TRHergic PVN neurons caused by DIA. These new data further supports clinical trials with CRH-R2 antagonists in anorexia nervosa patients.
Keyword Trh
Thyroid axis
Anorexia nervosa
Antisauvagine-30
Pvn
Corticotropin-Releasing-Factor
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Agriculture and Food Sciences
 
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Created: Mon, 18 Jul 2016, 08:26:33 EST by Karen Mancera Alarcon on behalf of Learning and Research Services (UQ Library)