The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications

Nhu, Nguyen Thi Khanh, Riordan, David W., Nhu, Tran Do Hoang, Thanh, Duy Pham, Thwaites, Guy, Lan, Nguyen Phu Huong, Wren, Brendan W., Baker, Stephen and Stabler, Richard A. (2016) The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications. Scientific Reports, 6 . doi:10.1038/srep28291


Author Nhu, Nguyen Thi Khanh
Riordan, David W.
Nhu, Tran Do Hoang
Thanh, Duy Pham
Thwaites, Guy
Lan, Nguyen Phu Huong
Wren, Brendan W.
Baker, Stephen
Stabler, Richard A.
Title The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications
Formatted title
The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-06-22
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep28291
Open Access Status DOI
Volume 6
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
Acinetobacter baumannii is a significant cause of opportunistic hospital acquired infection and has been identified as an important emerging infection due to its high levels of antimicrobial resistance. Multidrug resistant A. baumannii has risen rapidly in Vietnam, where colistin is becoming the drug of last resort for many infections. In this study we generated spontaneous colistin resistant progeny (up to >256 μg/μl) from four colistin susceptible Vietnamese isolates and one susceptible reference strain (MIC <1.5 μg/μl). Whole genome sequencing was used to identify single nucleotide mutations that could be attributed to the reduced colistin susceptibility. We identified six lpxACD and three pmrB mutations, the majority of which were novel. In addition, we identified further mutations in six A. baumannii genes (vacJ, pldA, ttg2C, pheS and conserved hypothetical protein) that we hypothesise have a role in reduced colistin susceptibility. This study has identified additional mutations that may be associated with colistin resistance through novel resistance mechanisms. Our work further demonstrates how rapidly A. baumannii can generate resistance to a last resort antimicrobial and highlights the need for improved surveillance to identified A. baumannii with an extensive drug resistance profile.
Keyword Antimicrobial resistance
Bacterial genetics
Acinetobacter baumannii
Hospital acquired infection
Multidrug resistant
Drug resistance
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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