Detection of intracellular bacteria in exfoliated urothelial cells from women with urge incontinence

Cheng, Ying, Chen, Zhouran, Gawthorne, Jayde A., Mukerjee, Chinmoy, Varettas, Kerry, Mansfield, Kylie J., Schembri, Mark A. and Moore, Kate H. (2016) Detection of intracellular bacteria in exfoliated urothelial cells from women with urge incontinence. Pathogens and Disease, 74 7: . doi:10.1093/femspd/ftw067

Author Cheng, Ying
Chen, Zhouran
Gawthorne, Jayde A.
Mukerjee, Chinmoy
Varettas, Kerry
Mansfield, Kylie J.
Schembri, Mark A.
Moore, Kate H.
Title Detection of intracellular bacteria in exfoliated urothelial cells from women with urge incontinence
Journal name Pathogens and Disease   Check publisher's open access policy
ISSN 2049-632X
Publication date 2016-07-07
Year available 2016
Sub-type Article (original research)
DOI 10.1093/femspd/ftw067
Open Access Status Not Open Access
Volume 74
Issue 7
Total pages 19
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2017
Language eng
Formatted abstract
The role of subclinical infection in patients with urge incontinence has been largely ignored. The aim of this study was to test for the presence of intracellular bacteria in the urine of patients with detrusor overactivity or mixed incontinence +/- a history of UTI, and compare this to a control group of patients with stress incontinence and no history of infection. Bacterial cystitis was assessed by routine microbiology and compared to microscopic analysis of urine by Wright staining. Subsequent analysis of urothelial cells by confocal microscopy was performed to determine the existence of intracellular bacteria. Bacterial cystitis was seen in 13% of patients based on routine microbiology. Wright staining of concentrated urothelial cells demonstrated the presence of bacteria in 72% of samples. Filamentous bacterial cells were observed in 51% of patients and were significantly more common in patients with detrusor overactivity. Intracellular E. coli were observed by confocal microscopy. This study supports the possibility that a subset of patients with urge incontinence may have unrecognised chronic bacterial colonisation, maintained via an intracellular reservoir. In patients with negative routine microbiology, application of the techniques used in this study revealed evidence of infection, providing further insights into the aetiology of urge incontinence.
Keyword Bacterial cystitis
Intracellular bacteria
Recurrent urinary tract infection
Urge incontinence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
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Created: Fri, 15 Jul 2016, 11:24:21 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences