Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals

Ormerod, Kate L., Wood, David L. A., Lachner, Nancy, Gellatly, Shaan L., Daly, Joshua N., Parsons, Jeremy D., Dal’Molin, Cristiana G. O., Palfreyman, Robin W., Nielsen, Lars K., Cooper, Matthew A., Morrison, Mark, Hansbro, Philip M. and Hugenholtz, Philip (2016) Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals. Microbiome, 4 36.1-36.17. doi:10.1186/s40168-016-0181-2


Author Ormerod, Kate L.
Wood, David L. A.
Lachner, Nancy
Gellatly, Shaan L.
Daly, Joshua N.
Parsons, Jeremy D.
Dal’Molin, Cristiana G. O.
Palfreyman, Robin W.
Nielsen, Lars K.
Cooper, Matthew A.
Morrison, Mark
Hansbro, Philip M.
Hugenholtz, Philip
Title Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals
Formatted title
Genomic characterization of the uncultured Bacteroidales family S24-7 inhabiting the guts of homeothermic animals
Journal name Microbiome   Check publisher's open access policy
ISSN 2049-2618
Publication date 2016-07-07
Sub-type Article (original research)
DOI 10.1186/s40168-016-0181-2
Open Access Status DOI
Volume 4
Start page 36.1
End page 36.17
Total pages 17
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2017
Language eng
Formatted abstract
Background: Our view of host-associated microbiota remains incomplete due to the presence of as yet uncultured constituents. The Bacteroidales family S24-7 is a prominent example of one of these groups. Marker gene surveys indicate that members of this family are highly localized to the gastrointestinal tracts of homeothermic animals and are increasingly being recognized as a numerically predominant member of the gut microbiota; however, little is known about the nature of their interactions with the host.

Results: Here, we provide the first whole genome exploration of this family, for which we propose the name “Candidatus Homeothermaceae,” using 30 population genomes extracted from fecal samples of four different animal hosts: human, mouse, koala, and guinea pig. We infer the core metabolism of “Ca. Homeothermaceae” to be that of fermentative or nanaerobic bacteria, resembling that of related Bacteroidales families. In addition, we describe three trophic guilds within the family, plant glycan (hemicellulose and pectin), host glycan, and α-glucan, each broadly defined by increased abundance of enzymes involved in the degradation of particular carbohydrates.

Conclusions: “Ca. Homeothermaceae” representatives constitute a substantial component of the murine gut microbiota, as well as being present within the human gut, and this study provides important first insights into the nature of their residency. The presence of trophic guilds within the family indicates the potential for niche partitioning and specific roles for each guild in gut health and dysbiosis.
Keyword Gut microbiome
S24-7
Homeothermaceae
Population genomes
Metagenomics
Comparative genomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Tue, 12 Jul 2016, 12:32:27 EST by Dr Cristiana Dal'molin on behalf of School of Chemical Engineering