Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM

Aston, Robyn, Sewell, Kim, Klein, Travis, Lawrie, Gwen and Grondahl, Lisbeth (2016) Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM. European Polymer Journal, 82 1-15. doi:10.1016/j.eurpolymj.2016.06.025


Author Aston, Robyn
Sewell, Kim
Klein, Travis
Lawrie, Gwen
Grondahl, Lisbeth
Title Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM
Journal name European Polymer Journal   Check publisher's open access policy
ISSN 1873-1945
0014-3057
Publication date 2016-07-28
Sub-type Article (original research)
DOI 10.1016/j.eurpolymj.2016.06.025
Open Access Status Not Open Access
Volume 82
Start page 1
End page 15
Total pages 15
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2017
Language eng
Abstract The structural characterisation of high water content materials such as hydrogels is challenging due to the inherent properties of water that are dependent on conditions of temperature and pressure. Cryo-scanning electron microscopy (cryo SEM) has potential to offer the most authentic insights into the native structure of polysaccharide hydrogels, due to the ability to freeze the sample under conditions where transparent vitreous ice is formed. The advantages, challenges and constraints of cryo-SEM in characterising the porous matrix of alginate hydrogels are considered in this tutorial paper through examples from our own studies. Recommendations are proposed for the minimum methodological information that is required from researchers as part of the publication of their studies of hydrogel samples to enable critical appraisal of their cryo-SEM images.
Keyword Hydrogel
Cryo-SEM
Structural characterisation
Cryo-fixation
Pore size
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
Centre for Microscopy and Microanalysis Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 12 Jul 2016, 12:31:57 EST by Kim Sewell on behalf of Centre for Microscopy and Microanalysis