The chemistry of the silver precipitation method used for the histochemical localizgtion of ascorbic acid

Willis R.J. and Kratzing C.C. (1974) The chemistry of the silver precipitation method used for the histochemical localizgtion of ascorbic acid. Biotechnic and Histochemistry, 49 6: 381-386. doi:10.3109/10520297409117015


Author Willis R.J.
Kratzing C.C.
Title The chemistry of the silver precipitation method used for the histochemical localizgtion of ascorbic acid
Journal name Biotechnic and Histochemistry   Check publisher's open access policy
ISSN 1052-0295
Publication date 1974
Sub-type Article (original research)
DOI 10.3109/10520297409117015
Volume 49
Issue 6
Start page 381
End page 386
Total pages 6
Publisher Informa Healthcare
Subject 2722 Histology
3607 Medical Laboratory Technology
Abstract The usefulness of reductive silver precipitation for the histo chemical localization of assorbic acid in mouse lung has been examined under standard conditions. The estimated stoichiometry of the reaction shows that only one quarter of the silver precipitated in fresh tissue by the silver nitrate reagent was due to ascorbic acid. Although no evidence was found that diffusion of ascorbic acid proceeded more rapidly than reductive silver precipitation in the tissue, diffusion artifacts at the cellular led would seem likely to occur. Thiosulphate was shown to be more effective than ammonia m removing un reacted silver from tissues treated with silver nitrate.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 12 Jul 2016, 03:28:05 EST by System User