Experimental tests of charge conservation in macromolecular interactions

Ford C.L. and Winzor D.J. (1983) Experimental tests of charge conservation in macromolecular interactions. BBA - General Subjects, 756 1: 49-55. doi:10.1016/0304-4165(83)90023-5

Author Ford C.L.
Winzor D.J.
Title Experimental tests of charge conservation in macromolecular interactions
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1983-03-15
Sub-type Article (original research)
DOI 10.1016/0304-4165(83)90023-5
Volume 756
Issue 1
Start page 49
End page 55
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
2700 Medicine
Abstract A combination of equilibrium dialysis and ultrafiltration has been used to demonstrate the conservation of charge in the interaction between bovine serum albumin and methyl orange in Tris-HCl buffer, pH 7.4, I = 0.05 M; and also in the dimerization of α-chymotrypsin in acetate/chloride buffer, pH 3.9, I = 0.11 M, containing various concentrations of indole (0-10 mM) in order to displace the equilibrium position towards monomer. In the former study the magnitude of the negative charge on the albumin was shown to increase linearly with the number of molecules of methyl orange bound to the protein, the observed slope (0.96 ± 0.08) of this relationship being in excellent agreement with that predicted on the basis of charge conservation for attachment of the univalent, negatively charged methyl orange ligand. In the study of α-chymotrypsin, the net charge (expressed per monomeric enzyme unit) was + 10 in solutions in which the mole fraction of monomer varied between 0.47 and 0.88, the extent of this range having been established by means of constituent association equilibrium constant obtained from sedimentation equilibrium studies.
Keyword Albumin-dye interacion
Charge conservation
Equilbrium dialysis
Protein valence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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