Functional expression cloning reveals proapoptotic role for protein phosphatase 4

Mourtada-Maarabouni, M., Kirkham, L., Jenkins, B., Rayner, J., Gonda, T. J., Starr, R., Trayner, I., Farzaneh, F. and Williams, G. T. (2003) Functional expression cloning reveals proapoptotic role for protein phosphatase 4. Cell Death and Differentiation, 10 9: 1016-1024. doi:10.1038/sj.cdd.4401274


Author Mourtada-Maarabouni, M.
Kirkham, L.
Jenkins, B.
Rayner, J.
Gonda, T. J.
Starr, R.
Trayner, I.
Farzaneh, F.
Williams, G. T.
Title Functional expression cloning reveals proapoptotic role for protein phosphatase 4
Journal name Cell Death and Differentiation   Check publisher's open access policy
ISSN 1350-9047
1476-5403
Publication date 2003
Sub-type Article (original research)
DOI 10.1038/sj.cdd.4401274
Volume 10
Issue 9
Start page 1016
End page 1024
Total pages 9
Place of publication London, United Kingdom
Publisher Nature
Language eng
Abstract Functional expression cloning strategies are highly suitable for the analysis of the molecular control of apoptosis. This approach has two critical advantages. Firstly, it eliminates prior assumptions about the properties of the proteins involved, and, secondly, it selectively targets proteins that are causally involved in apoptosis control and which affect the crucial cellular decision between survival and death. The application of this strategy to the isolation of cDNAs conferring resistance to dexamethasone and gamma-irradiation resulted in the isolation of a partial cDNA for the catalytic subunit of protein phosphatase 4 (PP4). Cells transfected with this partial cDNA in an expression vector downregulated PP4 and were resistant to both dexamethasone and UV radiation, as demonstrated by both membrane integrity and colony-forming assays. These observations suggest that PP4 plays an important proapoptotic role in T lymphocytes.
Keyword Apoptosis
Dephosphorylation
Phosphatase
PP4
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Mon, 13 Aug 2007, 13:45:58 EST