Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Uddin, Muhammad Jasim, Suen, Willy W., Bosco-Lauth, Angela, Hartwig, Airn-Elizabeth, Hall, Roy A., Bowen, Richard A. and Bielefeldt-Ohmann, Helle (2016) Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells. Veterinary Research, 47 . doi:10.1186/s13567-016-0347-8


Author Uddin, Muhammad Jasim
Suen, Willy W.
Bosco-Lauth, Angela
Hartwig, Airn-Elizabeth
Hall, Roy A.
Bowen, Richard A.
Bielefeldt-Ohmann, Helle
Title Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells
Journal name Veterinary Research   Check publisher's open access policy
ISSN 0928-4249
1297-9716
Publication date 2016-06
Year available 2016
Sub-type Article (original research)
DOI 10.1186/s13567-016-0347-8
Open Access Status DOI
Volume 47
Total pages 16
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2017
Language eng
Formatted abstract
West Nile virus (WNV) is one of the most common causes of epidemic viral encephalitis in horses worldwide. Peripheral blood mononuclear cells (PBMCs) are amongst the first to encounter the virus following a mosquito bite. This study aimed to elucidate the transcription kinetics of cytokine, Toll-like receptor (TLRs) and TLRs-associated genes following WNV challenge of equine PBMCs. PBMCs were challenged with an Australian strain of WNV (WNVNSW2011) and transcriptomes were quantified at 2, 6, 12 and 24 h post-infection (pi) using qRT-PCR. Type I and II interferons (IFNα, β and γ) mRNA transcription increased following WNV exposure, as did the transcripts for IL1α, IL1β, IL6, IL8, and IL22, but with slightly varying kinetics. TLR1, 3, 5, 7-9 transcripts were also upregulated in equine PBMCsin response to WNV challenge, as were those for MyD88, NF-κB, TRAF3, STAT1 and 2, IRF3 and 7, ISG15, as well as SOCS1 and 3 compared to the control cells. Expression of selected genes in the draining lymph node, spleen and brain (medulla oblongata) of experimentally infected horses was also assessed and transcription of most of these genes was also upregulated here. Although qRT-PCR detected higher viral RNA at 24 h pi compared to 6 h pi, the virus did not replicate productively in equine PBMCs. The up-regulation of gene-transcription for selected cytokines, IFNs, TLRs and TLRs-associated molecules suggests their involvement in virus recognition and control of WNV infection in the horse.
Keyword Innate immune control
Messenger RNA
Encephalitis virus
Gene expression
Ikk Epsilon
Rt-Pcr
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
School of Veterinary Science Publications
 
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