Transformation of Stylosanthes spp. using Agrobacterium tumefaciens

Manners J.M. (1987) Transformation of Stylosanthes spp. using Agrobacterium tumefaciens. Plant Cell Reports, 6 3: 204-207. doi:10.1007/BF00268480


Author Manners J.M.
Title Transformation of Stylosanthes spp. using Agrobacterium tumefaciens
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1987
Sub-type Article (original research)
DOI 10.1007/BF00268480
Volume 6
Issue 3
Start page 204
End page 207
Total pages 4
Publisher Springer-Verlag
Subject 1110 Nursing
Abstract Tumours were incited on leaf sections of Stylosanthes humilis, S. hamata, S. guianensis and S. scalra following infection by Agrobacterium tumefaciens. The suitability of 2 binary vectors (pGA472, BIN6) for gene transfer in S. humilis was tested and kanamycin-resistant tumour tissue was obtained from infected leaf pieces. The presence and expression of the neomycin phosphotransferase (NPT II) gene in the plant cells was demonstrated by hybridization of the coding region of the NPT II gene of the transposon Tn5 to DNA and RNA of kanamycin resistant tumours and by detection of significant NPT II activity in tissue extracts. Tumours also produced teratomatous shoots expressing the NPT II gene, but these could not be rooted.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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