The post-mortem stability of certain oxidative enzymes in brain and spinal cord

Tyrer J.H., Eadie M.J. and Kukums J.R. (1971) The post-mortem stability of certain oxidative enzymes in brain and spinal cord. Histochemie, 27 1: 21-27. doi:10.1007/BF00263364


Author Tyrer J.H.
Eadie M.J.
Kukums J.R.
Title The post-mortem stability of certain oxidative enzymes in brain and spinal cord
Journal name Histochemie   Check publisher's open access policy
ISSN 0948-6143
Publication date 1971
Sub-type Article (original research)
DOI 10.1007/BF00263364
Volume 27
Issue 1
Start page 21
End page 27
Total pages 7
Publisher Springer-Verlag
Subject 3105 Instrumentation
1307 Cell Biology
2700 Medicine
Abstract An investigation has been carried out on the stability of several enzymes in portions of rabbit brain and spinal cord kept at controlled temperatures between 22 and 37° C for periods up to 24 hours before processing for enzyme activity. The enzymes studied were NAD diaphorase, succinate, lactate, glutamate and glucose-6-phosphate dehydrogenases, and monoamine oxidase. One-wavelength "plug" cytophotometric measurements of enzyme activity were carried out on Purkinje cells, neuropil of the granular layer of the cerebellar cortex and on anterior horn cells. Succinate dehydrogenase activity proved to be stable after 24 hours post-mortem exposure at 37°C. Lactate dehydrogenase, NAD diaphorase and monoamine oxidase activities were less stable at the higher temperatures but were stable at 22°C. Glutamate and glucose-6-phosphate dehydrogenase activities fell significantly with exposure at 22°C. It thus appears possible to make valid histochemical measurements of the activities of certain oxidative enzymes in selected post-mortem brain material.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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