Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay

Johnson L.A., Gordon R.B. and Emmerson B.T. (1977) Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay. Clinica Chimica Acta, 80 1: 203-207. doi:10.1016/0009-8981(77)90280-7


Author Johnson L.A.
Gordon R.B.
Emmerson B.T.
Title Hypoxanthine-guanine phosphoribosyltransferase: A simple spectrophotometric assay
Journal name Clinica Chimica Acta   Check publisher's open access policy
ISSN 0009-8981
Publication date 1977-10-01
Sub-type Article (original research)
DOI 10.1016/0009-8981(77)90280-7
Volume 80
Issue 1
Start page 203
End page 207
Total pages 5
Subject 1303 Specialist Studies in Education
1308 Clinical Biochemistry
Abstract A simple spectrophotometric assay is described based on the conversion of hypoxanthine to inosine monophosphate and precipitation of both the reaction product and protein with lanthanum phosphate. The extent of conversion is determined by the fall in absorbance of hypoxanthine at 249 nm. The assay is suitable for screening red cell lysates for hypoxanthine-guanine phosphoribosyl-transferase deficiency.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Sat, 09 Jul 2016, 05:58:04 EST by System User