In vitro partial relipidation of apolipoproteins in plasma

Cham, B. Elliot and Knowles, Brian R. (1976) In vitro partial relipidation of apolipoproteins in plasma. Journal of Biological Chemistry, 251 20: 6367-6371.

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Author Cham, B. Elliot
Knowles, Brian R.
Title In vitro partial relipidation of apolipoproteins in plasma
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 1976-10-25
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 251
Issue 20
Start page 6367
End page 6371
Total pages 5
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Abstract In vitro recombination of lipids with apolipoproteins is achieved when a concentrated solution of plasma lipids in petroleum ether is mixed with delipidated plasma. Combination of phospholipids and unesterified fatty acids are observed in amounts comparable with those originally present in the native unextracted plasma; triglycerides combine partially and cholesterol only slightly. On agarose gel immunoelectrophoresis, a component in the delipidated plasma which is reactive with high density lipoprotein antibodies migrates more slowly than high density lipoprotein in the undelipidated control plasma. However, the component reacting with low density lipoprotein antibodies in the delipidated plasma moves more rapidly than low density lipoprotein in the native plasma. These changes are reversed by recombination of lipid with delipidated plasma. All lipids present in the plasma phase after relipidation travel with the lipoproteins during zonal electrophoresis. The apparent concentrations of proteins reacting with high density and low density lipoprotein antibodies decrease when no lipid is present in plasma on assay by single radial immunodiffusion and immunoelectrophoresis, using commercially available lipoprotein antibodies. On relipidation, full immunochemical properties of high density lipoprotein are restored, but relipidated low density lipoprotein exhibits only partial immunochemical restoration.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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