A method for the stabilisation of recombinant plasmids in yeast

Caunt P., Impoolsup A. and Greenfield P.F. (1990) A method for the stabilisation of recombinant plasmids in yeast. Journal of Biotechnology, 14 3-4: 311-320. doi:10.1016/0168-1656(90)90115-R


Author Caunt P.
Impoolsup A.
Greenfield P.F.
Title A method for the stabilisation of recombinant plasmids in yeast
Journal name Journal of Biotechnology   Check publisher's open access policy
ISSN 0168-1656
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1016/0168-1656(90)90115-R
Volume 14
Issue 3-4
Start page 311
End page 320
Total pages 10
Subject 1305 Biotechnology
Abstract The stability of a yeast plasmid can be improved using deliberately induced cyclic changes in the dissolved oxygen tension (DOT), during continuous culture in a non-selective, undefined medium. The resultant stability of the plasmid under DOT cycled conditions is strongly dependent on the growth rate of the culture, with complete stabilisation at lower growth rates. We propose a mechanism for the stabilisation and suggest that the method can be applied to other recombinant yeast strains.
Keyword Dissolved oxygen tension cycling
Non-selective medium
Recombinant plasmid
Saccharomyces cerevisiae
Stability
Yeast
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 05 Jul 2016, 14:12:05 EST by System User