Detection of polyols and sugars by cuprammonium ion in the presence of strong base

McKay D.B., Tanner G.P., Maclean D.J. and Scott K.J. (1987) Detection of polyols and sugars by cuprammonium ion in the presence of strong base. Analytical Biochemistry, 165 2: 392-398. doi:10.1016/0003-2697(87)90287-9


Author McKay D.B.
Tanner G.P.
Maclean D.J.
Scott K.J.
Title Detection of polyols and sugars by cuprammonium ion in the presence of strong base
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1987
Sub-type Article (original research)
DOI 10.1016/0003-2697(87)90287-9
Volume 165
Issue 2
Start page 392
End page 398
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A new and more sensitive cuprammonium reagent containing strong base has been developed for the colorimetric detection of polyols and sugars. A reagent blank with an absorbance at 280 nm, less than half that of previous methods, was achieved by reducing the cupric ion and ammonia concentrations to 1 mm (from 5 mm) and 0.4 m (from 2.25 m), respectively, and adding 0.2 m sodium hydroxide. Polyols are particularly well suited to the new method, showing up to an eightfold increase in sensitivity. The new reagent is readily applicable to the postcolumn detection of polyols and sugars after high-performance liquid chromatography and is linear over the range 40-180 nmol for d-glucitol (sorbitol) and 10-300 nmol for glucose, using standard HPLC equipment.
Keyword carbohydrates
cuprammonium
HPLC of sugars
polyols
sorbitol
sugar alcohols
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 05 Jul 2016, 03:26:11 EST by System User