Heterogeneity and molecular weight inter-relationships of the esterase isoenzymes of several invertebrate species

Haites N., Don M. and Masters C.J. (1972) Heterogeneity and molecular weight inter-relationships of the esterase isoenzymes of several invertebrate species. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 42 2: 303-322. doi:10.1016/0305-0491(72)90275-1


Author Haites N.
Don M.
Masters C.J.
Title Heterogeneity and molecular weight inter-relationships of the esterase isoenzymes of several invertebrate species
Journal name Comparative Biochemistry and Physiology -- Part B: Biochemistry and   Check publisher's open access policy
ISSN 0305-0491
Publication date 1972-06-15
Sub-type Article (original research)
DOI 10.1016/0305-0491(72)90275-1
Volume 42
Issue 2
Start page 303
End page 322
Total pages 20
Subject 2700 Medicine
Abstract 1. 1. The multiple forms of esterase activity have been studied in the tissues of eight representative invertebrate species: the sea anemone, the liver fluke, the round worm, the segmented worm, the king prawn, the garden snail, the chiton and the sea cucumber. 2. 2. These esterases have been characterized in terms of their relative mobilities on polyacrylamide gel electrophoresis, substrate and inhibitor specificity, molecular weights and physicochemical properties. 3. 3. In the representative of the phylum Coelenterata, the sea anemone, eleven esterase multiple forms were characterized and included carboxylesterases and cholinesterases. In the liver fluke (phylum Platyhelminthes) eight esterases were distinguishable. All of these were again carboxylesterases and cholinesterases. 4. 4. Thirteen multiple forms of esterases were found in the round worm of the phylum Nematoda, while only ten were present in the representative of the phylum Annelida, the earth worm. Both these species contained carboxylesterase, cholinesterase and arylesterase forms. 5. 5. The most complex system was found in the tissues of the king prawn (phylum Arthropoda) with twenty-nine isoenzymes. In the garden snail, eighteen isoenzymes were found, but in the marine mollusc, the chiton, only eleven forms were distinguishable. All these species contain the three types of esterases classified in the round worm and the earth worm. 6. 6. In the sea cucumber, from the phylum Echinodermata, only five carboxylesterases were found. 7. 7. These results have been discussed in relation to the phylogenetic variability of the esterases, and the complex structural and genetic inter-relationships of these multiple enzyme forms.
Keyword classification
electrophoresis
esterase
invertebrates
molecular weights
Multiple forms
phylogenetic variability
physicochemical properties
specificity
structural and genetic inter-relationships
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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