The characterisation of piRNA-related 19mers in the mouse

Oey, Harald M., Youngson, Neil A. and Whitelaw, Emma (2011) The characterisation of piRNA-related 19mers in the mouse. BMC Genomics, 12 . doi:10.1186/1471-2164-12-315

Author Oey, Harald M.
Youngson, Neil A.
Whitelaw, Emma
Title The characterisation of piRNA-related 19mers in the mouse
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2011-06-15
Year available 2011
Sub-type Article (original research)
DOI 10.1186/1471-2164-12-315
Open Access Status DOI
Volume 12
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Piwi interacting RNA, or piRNA, is a class of small RNA almost exclusively expressed in the germline where they serve essential roles in retrotransposon silencing. There are two types, primary and secondary piRNA, and the latter is a product of enzymatic cleavage of retrotransposons' transcripts directed by the former. Recently, a new class of 19nt long RNA was discovered that is specific to testis and appears to be linked to secondary piRNA biogenesis.

Results: We locate clusters of the testis-specific 19mers, which we call piRNA-related 19mers (pr19RNA), and characterise the transcripts from which they are derived. Most pr19RNA clusters were associated with retrotransposons and unannotated antisense transcripts overlapping piRNA clusters. At these loci the abundance of 19mers was found to be greater than that of secondary piRNAs.

Conclusion: We find that pr19RNAs are distinguished from other RNA populations by their length and flanking sequence, allowing their identification without requiring overlapping piRNAs. Using such sequence features allows identification of the source transcripts, and we suggest that these likely represent the substrates of primary piRNA-guided RNA cleavage events. While pr19RNAs appear not to bind directly to Miwi or Mili, their abundance relative to secondary piRNAs, in combination with their precise length, suggests they may be more than by-products of secondary piRNA biogenesis.
Keyword PiRNA-related 19mers
Long interspersed nuclear elements
Intracisternal A particles
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Wed, 29 Jun 2016, 14:21:41 EST by Harald Oey on behalf of Learning and Research Services (UQ Library)