On the reversible adsorption of aldolase to a microsomal membrane fraction from rat brain

Clarke F.M. and Masters C.J. (1975) On the reversible adsorption of aldolase to a microsomal membrane fraction from rat brain. International Journal of Biochemistry, 6 2: 133-145. doi:10.1016/0020-711X(75)90115-9


Author Clarke F.M.
Masters C.J.
Title On the reversible adsorption of aldolase to a microsomal membrane fraction from rat brain
Journal name International Journal of Biochemistry   Check publisher's open access policy
ISSN 0020-711X
Publication date 1975
Sub-type Article (original research)
DOI 10.1016/0020-711X(75)90115-9
Volume 6
Issue 2
Start page 133
End page 145
Total pages 13
Subject 1303 Specialist Studies in Education
Abstract 1. 1. As an extension of previous studies on the adsorption of aldolase (EC 4.1.2.13) in rat brain, and the correlation between such adsorption and the expression of aldolase activity, the interaction between purified aldolase A4 and a microsomal membrane fraction which was the main locus of aldolase binding has been studied. 2. 2. The observed effects of pH, salts and metabolites in this binding support the proposition that electrostatic forces are of primary importance in the adsorption process, although more subtle interactions involving the active site of the enzyme are also indicated. 3. 3. The enzyme remained firmly bound to these membranes even after phospholipase and ribonuclease treatment, suggesting that the compounds removed by these enzymes were not primarily involved in the aldolase binding. It also became evident, however, that RNA possessed the ability to associate with either free or bound aldolase under the assay conditions employed, and that a correlation existed in whole brain homogenate between the degree of nucleic acid association with both the soluble and bound forms of the enzyme and expression of activity, rather than between enzyme adsorption and expression of activity as previously suggested. 4. 4. These phenomena are discussed in relation to the structural and metabolic features of this tissue.
Keyword adsorption
Aldolase
electrostatic forces
enzyme activity
microsomal membrane
nucleic acid
phospholipase
rat brain
ribonuclease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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