Metabolism of 2-deoxy-d-glucose by axenically grown mycelia of Puccinia graminis

Manners J.M., Maclean D.J. and Scott K.J. (1988) Metabolism of 2-deoxy-d-glucose by axenically grown mycelia of Puccinia graminis. Experimental Mycology, 12 4: 350-356. doi:10.1016/0147-5975(88)90026-6


Author Manners J.M.
Maclean D.J.
Scott K.J.
Title Metabolism of 2-deoxy-d-glucose by axenically grown mycelia of Puccinia graminis
Journal name Experimental Mycology   Check publisher's open access policy
ISSN 0147-5975
Publication date 1988-01-01
Sub-type Article (original research)
DOI 10.1016/0147-5975(88)90026-6
Volume 12
Issue 4
Start page 350
End page 356
Total pages 7
Subject 2402 Applied Microbiology and Biotechnology
Abstract To obtain information on the pathway(s) of assimilation of glucose by the wheat stem rust fungus (Puccinia graminis f. sp. tritici), axenically grown mycelia were incubated with the glucose analog 2-deoxy-d-[U-14C]glucose (2dG), and the kinetics of movement of label into intracellular metabolites was determined. During the first 5 minutes after adding substrate, free 2dG was transported into the cell, followed by phosphorylation. Further incubation over 5 h resulted in the accumulation of large pools of phosphorylated derivatives of 2dG and 2-deoxygluconate, and the accumulation of free dideoxytrehalose, 2-deoxyglucitol, 2-deoxygluconate, and traces of 2-deoxygalactose. A lag in the accumulation of 2-deoxyglucitol, without the prior accumulation of 2-deoxyglucitol phosphates, supports the view that glucitol is synthesized by direct reduction of free hexoses(s). The kinetics of labeling indicates that the major route of metabolism was oxidation of 2dG phosphate(s) to 2-deoxygluconate phosphate(s), from which free 2-deoxygluconate was released as a major end product; this is the first demonstration of this pathway of metabolism of 2dG in a fungus.
Keyword 2-deoxyglucitol
2-deoxygluconate
2-deoxyglucose transport and metabolism
dideoxytrehalose
rust fungi
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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