Vitamin K1 dependent carboxylase: β-Carboxylation of t-butyloxycarbonylaspartic acid α-benzyl ester

Hamilton S.E., Tesch D. and Zerner B. (1982) Vitamin K1 dependent carboxylase: β-Carboxylation of t-butyloxycarbonylaspartic acid α-benzyl ester. Biochemical and Biophysical Research Communications, 107 1: 246-249. doi:10.1016/0006-291X(82)91696-5


Author Hamilton S.E.
Tesch D.
Zerner B.
Title Vitamin K1 dependent carboxylase: β-Carboxylation of t-butyloxycarbonylaspartic acid α-benzyl ester
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1982-07-16
Sub-type Article (original research)
DOI 10.1016/0006-291X(82)91696-5
Volume 107
Issue 1
Start page 246
End page 249
Total pages 4
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Rat liver microsomes solubilized by Triton X-100 catalyze the vitamin K1 dependent incorporation of carbon-14 from [14C]NaHCO3 into t-butyloxycarbonylaspartic acid α-benzyl ester. High voltage electrophoresis of the alkaline hydrolysate of the products of this reaction demonstrates the presence of a labelled species (A) whose electrophoretic mobility is identical to that of β-carboxyaspartic acid. High voltage electrophoresis of the acid-treated products reveals the disappearance of A and the appearance of a labelled species whose electrophoretic mobility is identical to that of aspartic acid. These experiments provide unequivocal evidence for the vitamin K1 dependent β-carboxylation of an aspartic acid side chain, and they constitute the first report of such an enzymatic activity in microsomes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 28 Jun 2016, 05:06:56 EST by System User