Step elution in preparative liquid chromatography

Hu S.-G., Do D.D. and Hossain Md.M. (1992) Step elution in preparative liquid chromatography. Journal of Chromatography A, 605 2: 175-191. doi:10.1016/0021-9673(92)85235-L


Author Hu S.-G.
Do D.D.
Hossain Md.M.
Title Step elution in preparative liquid chromatography
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 1992-07-17
Sub-type Article (original research)
DOI 10.1016/0021-9673(92)85235-L
Volume 605
Issue 2
Start page 175
End page 191
Total pages 17
Subject 1602 Criminology
1308 Clinical Biochemistry
1313 Molecular Medicine
Abstract Preparative liquid chromatography with the step elution, in which a sample is loaded into a column at higher binding strength and then eluted at lower binding strength, was numerically simulated with a detailed rate equation model. The model takes into account film mass transfer resistance, pore diffusion, axial dispersion and local equilibrium. The step elution mode was compared with the isocratic elution mode in terms of the preparative performance for a binary mixture having a constant or variable separation factor. The effects of various parameters, such as step height, sample size, solute concentration, feed composition and mobile phase flow-rate, were examined.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 28 Jun 2016, 05:02:19 EST by System User