Immunoperoxidase staining characteristics of Dirofilaria immitis in the dog

Tanaka K.I. and Atwell R.B. (1991) Immunoperoxidase staining characteristics of Dirofilaria immitis in the dog. Research in Veterinary Science, 50 1: 33-37. doi:10.1016/0034-5288(91)90050-X


Author Tanaka K.I.
Atwell R.B.
Title Immunoperoxidase staining characteristics of Dirofilaria immitis in the dog
Journal name Research in Veterinary Science   Check publisher's open access policy
ISSN 0034-5288
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1016/0034-5288(91)90050-X
Volume 50
Issue 1
Start page 33
End page 37
Total pages 5
Subject 1103 Clinical Sciences
3400 Veterinary
Abstract The immunoperoxidase staining characteristics of Dirofilaria immitis and pulmonary tissues from infected dogs were studied by using the following sera: anti-fresh D immitis, anti-processed D immitis, anti-dog IgG, anti-dog IgG Fc, anti-dog IgM and antidog C3. Marked staining was observed using antifresh D immitis serum. Body cavity fluid and cuticle were strongly stained and hypodermis, muscle, lateral cord, testis, vas deferens, ovary, oviduct and uterus were moderately stained. Oesophagus and intestine were mildly stained. Degenerate worms were stained by all antisera. The intact and cut surfaces of microfilariae and eggs and sperm present in filariae were stained, but not their internal contents. Circulating and stored immotile microfilariae did not stain. Excreted eggs, presumed to be unfertilised and, or, degenerate, stained positively. Immunoperoxidase staining of routinely processed histological samples provides a means of assessing D immitis antigen.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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