Iron-containing acid phosphatases: Comparison of the enzymes from beef spleen and pig allantoic fluid

Campbell H.D., Dionysius D.A., Keough D.T., Wilson B.E., Jersey J.d. and Zerner B. (1978) Iron-containing acid phosphatases: Comparison of the enzymes from beef spleen and pig allantoic fluid. Biochemical and Biophysical Research Communications, 82 2: 615-620. doi:10.1016/0006-291X(78)90919-1


Author Campbell H.D.
Dionysius D.A.
Keough D.T.
Wilson B.E.
Jersey J.d.
Zerner B.
Title Iron-containing acid phosphatases: Comparison of the enzymes from beef spleen and pig allantoic fluid
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1978-05-30
Sub-type Article (original research)
DOI 10.1016/0006-291X(78)90919-1
Volume 82
Issue 2
Start page 615
End page 620
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The iron-containing violet acid phosphatases from beef spleen and pig allantoic fluid have been purified to homogeneity. Molecular weight determinations by zonal gel filtration, SDS-gel electrophoresis, and ultracentrifugation support values close to 40,000 for both enzymes, necessitating reappraisal of literature values. Similarly, the equivalent weight for iron is close to 20,000 for both enzymes, indicating the presence of two iron atoms per molecule of enzyme. The enzymes also have very similar ultraviolet and visible spectra, with λmax values close to 550 nm, and ε{lunate}550 values(in terms of iron) of 2.04 × 103 and 2.00 × 103 for the beef spleen and pig allantoic fluid enzymes respectively.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 65 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 28 Jun 2016, 14:39:52 EST by System User