A mouse partially protective glycoprotein antigen in Streptococcus agalactiae serotype Ia

Somerset S.M. and Davis G.H.G. (1986) A mouse partially protective glycoprotein antigen in Streptococcus agalactiae serotype Ia. Current Microbiology, 14 4: 213-216. doi:10.1007/BF01568520


Author Somerset S.M.
Davis G.H.G.
Title A mouse partially protective glycoprotein antigen in Streptococcus agalactiae serotype Ia
Journal name Current Microbiology   Check publisher's open access policy
ISSN 0343-8651
Publication date 1986
Sub-type Article (original research)
DOI 10.1007/BF01568520
Volume 14
Issue 4
Start page 213
End page 216
Total pages 4
Publisher Springer-Verlag
Subject 2404 Microbiology
Abstract The immunological properties of a glycoprotein antigen (antigen 2) of Streptococcus agalactiae serotype Ia were investigated. A specific antiserum was prepared by immunizing rabbits with antigen 2 immunoprecipitates excised from Crossed immunoelectrophoresis (Crossed IEP) gels. This antiserum produced a single peak representing antigen 2 when reacted with a Triton X-100 sonicate of heat-killed whole serotype Ia cells in Crossed IEP analysis. With polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and subsequent immunoelectroblotting, three strongly reacting polypeptides were detected at 60, 56, and 35 kilo-Daltons. Many faintly reacting polypeptides were detected between 67 and 30 kilodalton. The specific anti-antigen 2 serum used in Crossed immunoisoelectric focusing (XIEF) detected three immunoprecipitates, two with a pI of 8.4 and one with a pI of 6.7. Identification of the antigens detected in XIEF with the polypeptides detected by immunoelectroblotting was not attempted. The specific anti-antigen 2 serum partially protected mice against lethal serotype Ia infection.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 28 Jun 2016, 04:24:36 EST by System User