Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production

Hoppner T.C. and Doelle H.W. (1983) Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production. European Journal of Applied Microbiology and Biotechnology, 17 3: 152-157. doi:10.1007/BF00505880


Author Hoppner T.C.
Doelle H.W.
Title Purification and kinetic characteristics of pyruvate decarboxylase and ethanol dehydrogenase from Zymomonas mobilis in relation to ethanol production
Journal name European Journal of Applied Microbiology and Biotechnology
ISSN 0171-1741
Publication date 1983
Sub-type Article (original research)
DOI 10.1007/BF00505880
Volume 17
Issue 3
Start page 152
End page 157
Total pages 6
Publisher Springer-Verlag
Subject 2404 Microbiology
1305 Biotechnology
2700 Medicine
Abstract Alcohol dehydrogenase (EC 1.1.1.1) and pyruvate decarboxylase (EC 4.1.1.1) from Zymomonas mobilis were partially purified and characterized. Alcohol dehydrogenase exhibits a pH optimum at 6.5 and pyruvate decarboxylase a major peak at pH 6.0 and a minor one at pH 4.3. The molecular weights were estimated to be 147,300±14,700 and 219,700±20,400 daltons, respectively. Both enzymes exhibit hyperbolic saturation curves with their respective substrates. Whereas alcohol dehydrogenase was inhibited by ethanol (Ki=6.86×10-4 M) and NAD+ (Ki=1.44×10-4 M), no inhibition was observed with pyruvate decarboxylase at similar concentrations of ethanol. Neither of the enzymes responded to sulphydryl-binding reagents, but differed in their response to a number of divalent metals. The results are compared with those of the respective enzymes from yeast and discussed with a view towards ethanol production limitations by Zymomonas mobilis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 69 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 28 Jun 2016, 03:31:41 EST by System User