Effect of novobiocin on DNA synthesis and structure in human lymphoblastoid cells

Lavin M.F. (1981) Effect of novobiocin on DNA synthesis and structure in human lymphoblastoid cells. Biochemical and Biophysical Research Communications, 100 1: 328-335. doi:10.1016/S0006-291X(81)80100-3


Author Lavin M.F.
Title Effect of novobiocin on DNA synthesis and structure in human lymphoblastoid cells
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1981-05-15
Sub-type Article (original research)
DOI 10.1016/S0006-291X(81)80100-3
Volume 100
Issue 1
Start page 328
End page 335
Total pages 8
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Novobiocin has an inhibitory effect on DNA synthesis in human lymphoblastoid cells similar to that observed with other mammalian cells. A concentration and time-dependent increase in sedimentation of DNA nucleoids, derived from human lymphoblastoid cells, was observed in the presence of novobiocin. No decrease in the degree of negative supercoiling of DNA, determined by sedimentation on sucrose gradients in the presence of ethidium bromide, was obtained when cells were incubated with novobiocin. The latter findings are contrary to results obtained with other mammalian cells. Alteration in sedimentation rate of nucleoids after novobiocin treatment, at concentrations up to 0.25mg/ml, does not correlate with inhibition of DNA synthesis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 28 Jun 2016, 13:17:39 EST by System User