A recycling gel partition technique for the study of protein-ligand interactions: The binding of methyl orange to bovine serum albumin

Ford C.L. and Winzor D.J. (1981) A recycling gel partition technique for the study of protein-ligand interactions: The binding of methyl orange to bovine serum albumin. Analytical Biochemistry, 114 1: 146-152. doi:10.1016/0003-2697(81)90465-6


Author Ford C.L.
Winzor D.J.
Title A recycling gel partition technique for the study of protein-ligand interactions: The binding of methyl orange to bovine serum albumin
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1981
Sub-type Article (original research)
DOI 10.1016/0003-2697(81)90465-6
Volume 114
Issue 1
Start page 146
End page 152
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A recycling gel partition technique has been developed for binding studies with acceptor-ligand systems that exhibit suitable isosbestic points. Its use is illustrated with a study of the binding of methyl orange to bovine serum albumin in 0.1 m phosphate, pH 5.68, the results being in good agreement with those reported in the literature for this test system. A combination of binding studies and sedimentation velocity experiments has been used to dispel the concept (I. M. Klotz and J. M. Urquhart, 1949, J. Phys. Colloid Chem. 53, 100-114) that the binding curve for this system shows a dependence on the concentration of bovine serum albumin used for its measurement.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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