Genome-centric analysis of microbial populations enriched by hydraulic fracture fluid additives in a coal bed methane production well

Robbins, Steven J., Evans, Paul N., Parks, Donovan H., Golding, Suzanne D. and Tyson, Gene W. (2016) Genome-centric analysis of microbial populations enriched by hydraulic fracture fluid additives in a coal bed methane production well. Frontiers in Microbiology, 7 731: 1-15. doi:10.3389/fmicb.2016.00731


Author Robbins, Steven J.
Evans, Paul N.
Parks, Donovan H.
Golding, Suzanne D.
Tyson, Gene W.
Title Genome-centric analysis of microbial populations enriched by hydraulic fracture fluid additives in a coal bed methane production well
Journal name Frontiers in Microbiology   Check publisher's open access policy
ISSN 1664-302X
Publication date 2016-06
Year available 2016
Sub-type Article (original research)
DOI 10.3389/fmicb.2016.00731
Open Access Status DOI
Volume 7
Issue 731
Start page 1
End page 15
Total pages 15
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2017
Language eng
Abstract Coal bed methane (CBM) is generated primarily through the microbial degradation of coal. Despite a limited understanding of the microorganisms responsible for this process, there is significant interest in developing methods to stimulate additional methane production from CBM wells. Physical techniques including hydraulic fracture stimulation are commonly applied to CBM wells, however the effects of specific additives contained in hydraulic fracture fluids on native CBM microbial communities are poorly understood. Here, metagenomic sequencing was applied to the formation waters of a hydraulically fractured and several non-fractured CBM production wells to determine the effect of this stimulation technique on the in-situ microbial community. The hydraulically fractured well was dominated by two microbial populations belonging to the class Phycisphaerae (within phylum Planctomycetes) and candidate phylum Aminicenantes. Populations from these phyla were absent or present at extremely low abundance in non-fractured CBM wells. Detailed metabolic reconstruction of near-complete genomes from these populations showed that their high relative abundance in the hydraulically fractured CBM well could be explained by the introduction of additional carbon sources, electron acceptors, and biocides contained in the hydraulic fracture fluid.
Keyword Coal bed methane
Aminicenantes
Op8
Phycisphaerae
Methane
Hydraulic fracturing
Metagenomics
Production Waters
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Earth Sciences Papers
School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Sun, 26 Jun 2016, 00:26:27 EST by System User on behalf of School of Chemistry & Molecular Biosciences