Lateral branching oxidoreductase acts in the final stages of strigolactone biosynthesis in Arabidopsis

Brewer, Philip B., Yoneyama, Kaori, Filardo, Fiona, Meyers, Emma, Scaffidi, Adrian, Frickey, Tancred, Akiyama, Kohki, Seto, Yoshiya, Dun, Elizabeth A., Cremer, Julia E., Kerr, Stephanie C., Waters, Mark T., Flematti, Gavin R., Mason, Michael G., Weiller, Georg, Yamaguchi, Shinjiro, Nomura, Takahito, Smith, Steven M., Yoneyama, Koichi and Beveridge, Christine A. (2016) Lateral branching oxidoreductase acts in the final stages of strigolactone biosynthesis in Arabidopsis. National Academy of Sciences. Proceedings, 113 22: 6301-6306. doi:10.1073/pnas.1601729113


Author Brewer, Philip B.
Yoneyama, Kaori
Filardo, Fiona
Meyers, Emma
Scaffidi, Adrian
Frickey, Tancred
Akiyama, Kohki
Seto, Yoshiya
Dun, Elizabeth A.
Cremer, Julia E.
Kerr, Stephanie C.
Waters, Mark T.
Flematti, Gavin R.
Mason, Michael G.
Weiller, Georg
Yamaguchi, Shinjiro
Nomura, Takahito
Smith, Steven M.
Yoneyama, Koichi
Beveridge, Christine A.
Title Lateral branching oxidoreductase acts in the final stages of strigolactone biosynthesis in Arabidopsis
Formatted title
Lateral branching oxidoreductase acts in the final stages of strigolactone biosynthesis in Arabidopsis
Journal name National Academy of Sciences. Proceedings   Check publisher's open access policy
ISSN 1091-6490
0027-8424
Publication date 2016-05-31
Year available 2016
Sub-type Article (original research)
DOI 10.1073/pnas.1601729113
Open Access Status Not Open Access
Volume 113
Issue 22
Start page 6301
End page 6306
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Collection year 2017
Language eng
Formatted abstract
Strigolactones are a group of plant compounds of diverse but related chemical structures. They have similar bioactivity across a broad range of plant species, act to optimize plant growth and development, and promote soil microbe interactions. Carlactone, a common precursor to strigolactones, is produced by conserved enzymes found in a number of diverse species. Versions of the MORE AXILLARY GROWTH1 (MAX1) cytochrome P450 from rice and Arabidopsis thaliana make specific subsets of strigolactones from carlactone. However, the diversity of natural strigolactones suggests that additional enzymes are involved and remain to be discovered. Here, we use an innovative method that has revealed a missing enzyme involved in strigolactone metabolism. By using a transcriptomics approach involving a range of treatments that modify strigolactone biosynthesis gene expression coupled with reverse genetics, we identified LATERAL BRANCHING OXIDOREDUCTASE (LBO), a gene encoding an oxidoreductase-like enzyme of the 2-oxoglutarate and Fe(II)-dependent dioxygenase superfamily. Arabidopsis lbo mutants exhibited increased shoot branching, but the lbo mutation did not enhance the max mutant phenotype. Grafting indicated that LBO is required for a graft-transmissible signal that, in turn, requires a product of MAX1. Mutant lbo backgrounds showed reduced responses to carlactone, the substrate of MAX1, and methyl carlactonoate (MeCLA), a product downstream of MAX1. Furthermore, lbo mutants contained increased amounts of these compounds, and the LBO protein specifically converts MeCLA to an unidentified strigolactone-like compound. Thus, LBO function may be important in the later steps of strigolactone biosynthesis to inhibit shoot branching in Arabidopsis and other seed plants.
Keyword Arabidopsis
Biosynthesis
Branching
Plant
Strigolactone
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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