Efficacy of three tourniquet types for intravenous antimicrobial regional limb perfusion in standing horses

Levine, David G., Epstein, Kira L., Ahern, Ben J. and Richardson, Dean W. (2010) Efficacy of three tourniquet types for intravenous antimicrobial regional limb perfusion in standing horses. Veterinary Surgery, 39 8: 1021-1024. doi:10.1111/j.1532-950X.2010.00732.x


Author Levine, David G.
Epstein, Kira L.
Ahern, Ben J.
Richardson, Dean W.
Title Efficacy of three tourniquet types for intravenous antimicrobial regional limb perfusion in standing horses
Journal name Veterinary Surgery   Check publisher's open access policy
ISSN 0161-3499
1532-950X
Publication date 2010-12
Sub-type Article (original research)
DOI 10.1111/j.1532-950X.2010.00732.x
Open Access Status Not yet assessed
Volume 39
Issue 8
Start page 1021
End page 1024
Total pages 4
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective: To determine (1) if clinically useful concentrations of amikacin sulfate can be obtained in synovial fluid during regional limb perfusion (RLP) performed above the carpus in standing sedated horses and (2) to determine the efficacy of 3 tourniquet types (narrow rubber [NR], wide rubber [WR], pneumatic [PN]).

Animals: Horses (n=9).

Methods: Bilateral forelimb RLP with amikacin sulfate (2.5 g) were administered through the cephalic vein in standing sedated horses. Limbs were randomly assigned to the 3 tourniquet types (NR, WR, PN) applied above the carpus. Metacarpophalangeal synovial fluid was obtained 0.5 hour after perfusion. Amikacin concentration in the synovial fluid was detected using fluorescence polarization immunoassay.

Results: Mean synovial concentrations of amikacin in the PN (mean 236 μg/mL; range 23-913 μg/mL) and WR (mean 64.2 μg/mL; range 7-315 μg/mL) were significantly higher (P=000 and .032, respectively) than the NR tourniquet (mean 2.1 μg/mL; range 0.9-3.3 μg/mL).

Conclusions: The PN tourniquet resulted in the highest synovial fluid amikacin concentrations in all horses, although administration with PN and WR tourniquets achieved adequate amikacin concentrations. NR tourniquet is ineffective and should not be used for RLP above the carpus in the standing horse.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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