The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization

Crichton, Michael Lawrence, Muller, David Alexander, Depelsenaire, Alexandra Christina Isobel, Pearson, Frances Elizabeth, Wei, Jonathan, Coffey, Jacob, Zhang, Jin, Fernando, Germain J. P. and Kendall, Mark Anthony Fernance (2016) The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization. Scientific Reports, 6 e27217.1-e27217.7. doi:10.1038/srep27217


Author Crichton, Michael Lawrence
Muller, David Alexander
Depelsenaire, Alexandra Christina Isobel
Pearson, Frances Elizabeth
Wei, Jonathan
Coffey, Jacob
Zhang, Jin
Fernando, Germain J. P.
Kendall, Mark Anthony Fernance
Title The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-06-02
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep27217
Open Access Status DOI
Volume 6
Start page e27217.1
End page e27217.7
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
Micro-device use for vaccination has grown in the past decade, with the promise of ease-of-use, painless application, stable solid formulations and greater immune response generation. However, the designs of the highly immunogenic devices (e.g. the gene gun, Nanopatch or laser adjuvantation) require significant energy to enter the skin (30–90 mJ). Within this study, we explore a way to more effectively use energy for skin penetration and vaccination. These modifications change the Nanopatch projections from cylindrical/conical shapes with a density of 20,000 per cm2 to flat-shaped protrusions at 8,000 per cm2, whilst maintaining the surface area and volume that is placed within the skin. We show that this design results in more efficient surface crack initiations, allowing the energy to be more efficiently be deployed through the projections into the skin, with a significant overall increase in penetration depth (50%). Furthermore, we measured a significant increase in localized skin cell death (>2 fold), and resultant infiltrate of cells (monocytes and neutrophils). Using a commercial seasonal trivalent human influenza vaccine (Fluvax 2014), our new patch design resulted in an immune response equivalent to intramuscular injection with approximately 1000 fold less dose, while also being a practical device conceptually suited to widespread vaccination.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Faculty of Medicine
Australian Institute for Bioengineering and Nanotechnology Publications
 
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