Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication

Baum, Oliver, Torchetti, Eleonora, Malik, Corinna, Hoier, Birgitte, Walker, Meegan, Walker, Philip J., Odriozola, Adolfo, Graber, Franziska, Tschanz, Stefan A., Bangsbo, Jens, Hoppeler, Hans, Askew, Christopher D. and Hellsten, Ylva (2016) Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 310 10: R943-R951. doi:10.1152/ajpregu.00480.2015


Author Baum, Oliver
Torchetti, Eleonora
Malik, Corinna
Hoier, Birgitte
Walker, Meegan
Walker, Philip J.
Odriozola, Adolfo
Graber, Franziska
Tschanz, Stefan A.
Bangsbo, Jens
Hoppeler, Hans
Askew, Christopher D.
Hellsten, Ylva
Title Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication
Journal name American Journal of Physiology-Regulatory Integrative and Comparative Physiology   Check publisher's open access policy
ISSN 0363-6119
1522-1490
Publication date 2016-05-15
Year available 2016
Sub-type Article (original research)
DOI 10.1152/ajpregu.00480.2015
Open Access Status Not Open Access
Volume 310
Issue 10
Start page R943
End page R951
Total pages 9
Place of publication Bethesda, MD, United States
Publisher American Physiological Society
Collection year 2017
Language eng
Formatted abstract
Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC patients and 10 age-matched controls, and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411 +/- 90 mm(-2) vs. 506 +/- 95 mm(-2); P <= 0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543 +/- 82 nm vs. 423 +/- 97 nm; P <= 0.01) and the volume density of mitochondria was lower (3.51 +/- 0.56% vs. 4.60 +/- 0.74%; P <= 0.01) in the IC patients than the controls. In the IC patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5 +/- 9.9Wvs. 33.5 +/- 9.4 W; P <= 0.01) in the IC patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC patients.
Keyword Capillary
Morphometry
Peripheral arterial disease
Skeletal muscle
Transmission electron microscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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