A novel osteotropic biomaterial OG-PLG: In vitro efficacy

Whang K., Grageda E., Khan A., McDonald J., Lawton M. and Satsangi N. (2005) A novel osteotropic biomaterial OG-PLG: In vitro efficacy. Journal of Biomedical Materials Research - Part A, 74 2: 247-253. doi:10.1002/jbm.a.30370


Author Whang K.
Grageda E.
Khan A.
McDonald J.
Lawton M.
Satsangi N.
Title A novel osteotropic biomaterial OG-PLG: In vitro efficacy
Journal name Journal of Biomedical Materials Research - Part A   Check publisher's open access policy
ISSN 0021-9304
Publication date 2005-08-01
Sub-type Article (original research)
DOI 10.1002/jbm.a.30370
Volume 74
Issue 2
Start page 247
End page 253
Total pages 7
Subject 2204 Religion and Religious Studies
2502 Biomaterials
Abstract Previously, a novel osteotropic biomaterial, OG-PLG [simvastatin grafted to poly(actide-co-glycolide), PLG], was synthesized and shown to have degradation-controlled release kinetics. The objective here was to determine the effect of grafting statins to PLG on bone regeneration in vitro. Rat bone marrow cells were stimulated in vitro with simvastatin dissolved in media, saponified simvastatin dissolved in media, simvastatin released through diffusion from emulsion freeze-dried scaffolds, and OG-PLG. Unstimulated cultures and cultures stimulated with dexamethasone were used as negative and positive controls, respectively. In vitro bone formation was assessed using the alkaline phosphatase (ALP) and von Kossa assays at different times up to 16 days. ALP analysis revealed that saponified simvastatin at 10-7 M and OG-PLG significantly increased ALP expression at various time points, von Kossa assay showed that simvastatin, saponified simvastatin, and OG-PLG significantly enhanced mineralization, with the effect from OG-PLG being the most significant. In short, OG-PLG significantly enhanced in vitro bone cell mineralization beyond the effect of simvastatin or saponified simvastatin dissolved in media and simvastatin released via diffusion from scaffolds.
Keyword Bone regeneration
Rat bone marrow cells
Simvastatin grafted to poly(lactide-co-glycolide) (OG-PLG)
Statin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Wed, 15 Jun 2016, 14:52:52 EST by Anthony Yeates