Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine

Sai, Linlin, Dong, Zhihua, Li, Ling, Guo, Qiming, Jia, Qiang, Xie, Lin, Bo, Cunxiang, Liu, Yanzhong, Qu, Binpeng, Li, Xiangxin, Shao, Hua, Ng, Jack C. and Peng, Cheng (2016) Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine. Chemosphere, 159 145-152. doi:10.1016/j.chemosphere.2016.05.008


Author Sai, Linlin
Dong, Zhihua
Li, Ling
Guo, Qiming
Jia, Qiang
Xie, Lin
Bo, Cunxiang
Liu, Yanzhong
Qu, Binpeng
Li, Xiangxin
Shao, Hua
Ng, Jack C.
Peng, Cheng
Title Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine
Formatted title
Gene expression profiles in testis of developing male Xenopus laevis damaged by chronic exposure of atrazine
Journal name Chemosphere   Check publisher's open access policy
ISSN 0045-6535
1879-1298
Publication date 2016-09
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.chemosphere.2016.05.008
Open Access Status Not Open Access
Volume 159
Start page 145
End page 152
Total pages 8
Place of publication Kidlington, Oxford United Kingdom
Publisher Pergamon Press
Collection year 2017
Language eng
Formatted abstract
As a widely used herbicide, atrazine (AZ) has been extensively studied for its adverse effects on the reproductive system, especially feminization in male animals. However, the relationship of gene expression changes and associated toxicological endpoints remains unclear. In this study, developing Xenopus laevis tadpoles were exposed to concentration of AZ at 0.1, 1, 10 or 100 μg/L continuously. Compared with froglets in the control group, there were no significant differences in body length, body weight, liver weight and hepatosomatic index (HSI) of males in groups treated with AZ for 90 d. At 100 μg/L AZ treatment caused a significant reduction of gonad weight and gonadosomatic index (GSI) of males (p < 0.01). In addition, AZ at all dose levels caused testicular degeneration, especially in froglets from the groups with 0.1 and 100 μg/L which exhibited U-shaped dose-response trend. We further investigated the gene expression changes associated with the testicular degeneration induced by AZ. We found that the expression of 1165 genes was significantly altered with 616 upregulated and 549 downregulated compared to the expression profile of the control animals. KEGG analysis showed that genes which were significantly affected by AZ are mainly involved in arginine and proline metabolism, cell cycle, riboflavin metabolism, spliceosome, base excision repair and progesterone-mediated oocyte maturation pathway. Our results show that AZ may affect reproductive and immune systems by interference with the related gene expression changes during the male X. laevis development. The findings may help to clarify the feminization mechanisms of AZ in male X. laevis.
Keyword Herbicide
Microarray
Reproductive system
Endocrine disruption
Frog
Tadpole
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
National Research Centre for Environmental Toxicology Publications
 
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Created: Tue, 14 Jun 2016, 13:10:49 EST by Dr Cheng Peng on behalf of National Res Centre For Environmental Toxicology