Studies of contact hypersensitivity and tolerance in vivo and in vitro: I. basic characteristics of the reactions and confirmation of an immune response in tolerant mice

Noonan F.P. and Halliday W.J. (1978) Studies of contact hypersensitivity and tolerance in vivo and in vitro: I. basic characteristics of the reactions and confirmation of an immune response in tolerant mice. International Archives of Allergy and Immunology, 56 6: 523-532. doi:10.1159/000232068


Author Noonan F.P.
Halliday W.J.
Title Studies of contact hypersensitivity and tolerance in vivo and in vitro: I. basic characteristics of the reactions and confirmation of an immune response in tolerant mice
Journal name International Archives of Allergy and Immunology   Check publisher's open access policy
ISSN 1423-0097
Publication date 1978
Sub-type Article (original research)
DOI 10.1159/000232068
Volume 56
Issue 6
Start page 523
End page 532
Total pages 10
Subject 2403 Immunology
2723 Immunology and Allergy
Abstract Contact hypersensitivity to dinitrochlorobenzene (DNCB), picryl chloride (PCI) or oxazolone was induced in mice by skin painting with these agents, and was measured by skin testing in vivo using the ear swelling method. Prior adminstration of dinitrobenzene sulfonate (DNBS) or picryl sulfonic acid prevented sensitization to DNCB and PCI, respectively. Both this tolerizaiion and the original sensitization were specific for the hapten used. Cyclophosphamide given before sensitization enhanced skin reactions, but when given before tolerization it interfered with establishment of tolerance. Cells from both sensitized and tolerized mice were shown to be reactive with the corresponding haptens in vitro in the leukocyte adherence inhibition (LAI) reaction. LAI specificity was similar to that found for cutaneous reactivity. The reaction of DNCB-sensitized cells with DNBS led to the production of a soluble mediator which induced LAI in normal cells. The demonstration of potentially reactive cells in mice judged to be tolerant by skin testing indicates the concomitant existence of suppressor factors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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