Appearance of three lymphokines in culture supernatants harvested at different times

Yong W.K. and Halliday W.J. (1982) Appearance of three lymphokines in culture supernatants harvested at different times. Immunology Letters, 4 1: 21-26. doi:10.1016/0165-2478(82)90072-4


Author Yong W.K.
Halliday W.J.
Title Appearance of three lymphokines in culture supernatants harvested at different times
Journal name Immunology Letters   Check publisher's open access policy
ISSN 0165-2478
Publication date 1982
Sub-type Article (original research)
DOI 10.1016/0165-2478(82)90072-4
Volume 4
Issue 1
Start page 21
End page 26
Total pages 6
Subject 2403 Immunology
2723 Immunology and Allergy
Abstract Peritoneal cells from mice with methylcholanthrene-induced tumours were reacted with the corresponding tumour extract (antigen) and supernatants were prepared after incubation times of 1, 3.5 and 24 h. All the supernatants were then tested simultaneously on the same pools of normal indicator cells for their effects on leucocyte adherence, E-rosette formation and macrophage migration. The leucocyte adherence inhibition factor (LAIF) was consistently detected only in the 1-h supernatant, E-rosette augmentation factor (E-RAF) only in the 3.5-h supernatant and migration inhibition factor (MIF) only in the 24-h supernatant. It is concluded that LAIF, E-RAF and MIF are probably distinct factors, having different stabilities to degradation or inhibition under the conditions of cell culture. A less likely interpretation is that a single factor with multiple activities is affected by a series of distinct inhibitors. In practical terms, the 3 assays correlate only if the appropriate incubation times are used.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Jun 2016, 06:47:25 EST by System User