Biospecific interactions: Their quantitative characterization and use for solute purification

Winzor D.J. and De Jersey J. (1989) Biospecific interactions: Their quantitative characterization and use for solute purification. Journal of Chromatography B: Biomedical Sciences and Applications, 492 C: 377-430. doi:10.1016/S0378-4347(00)84476-X


Author Winzor D.J.
De Jersey J.
Title Biospecific interactions: Their quantitative characterization and use for solute purification
Journal name Journal of Chromatography B: Biomedical Sciences and Applications
ISSN 0378-4347
Publication date 1989-08-08
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/S0378-4347(00)84476-X
Volume 492
Issue C
Start page 377
End page 430
Total pages 54
Subject 1600 Chemistry
1308 Clinical Biochemistry
1313 Molecular Medicine
Abstract Biospecificity is due largely to the formation and dissociation of non-covalent complexes between small molecules and macromolecules, or between two macromolecules. The first part of this review is concerned with the use of such biospecificity in the fractionation and identification of solutes. Major emphasis is given to affinity chromatography, especially in regard to the practical considerations inherent in an experimental situation and to the wide range of specific interactions that can be utilized. The second part of the review considers the quantitative characterization of biospecific complex formation. The merits of frontal gel chromatography, electrophoretic methods and affinity chromatography are discussed, and special consideration is given to the effects of ligand and/or acceptor multivalency because of its relevance to many biospecific interactions. Finally attention is drawn to the feasibility of employing quantitative affinity chromatographic theory for the determination of association constants for antigen-antibody systems by radioimmunoassay and enzyme-linked immunosorbent assay techniques.
Keyword A f-Valent solute (acceptor of ligand) K Equilibrium constant for solute self-association (molar scale)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: Scopus Import
 
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