Quantitation of oxycodone in human plasma using high–performance liquid chromatography with electrochemical detection

Smith M.T., Watt J.A., Mapp G.P. and Cramond T. (1991) Quantitation of oxycodone in human plasma using high–performance liquid chromatography with electrochemical detection. Therapeutic Drug Monitoring, 13 2: 126-130.

Author Smith M.T.
Watt J.A.
Mapp G.P.
Cramond T.
Title Quantitation of oxycodone in human plasma using high–performance liquid chromatography with electrochemical detection
Journal name Therapeutic Drug Monitoring   Check publisher's open access policy
ISSN 1536-3694
Publication date 1991
Sub-type Article (original research)
Volume 13
Issue 2
Start page 126
End page 130
Total pages 5
Subject 2736 Pharmacology (medical)
3004 Pharmacology
1300 Biochemistry, Genetics and Molecular Biology
1303 Specialist Studies in Education
2307 Health, Toxicology and Mutagenesis
2739 Public Health, Environmental and Occupational Health
3005 Toxicology
Abstract An original, highly sensitive and specific high-performance liquid chromatographic (HPLC) assay has been developed for the measurement of oxycodone in human plasma with a detection limit of 10 ng/ml using a 1.0-ml plasma sample. Plasma samples were initially acid-washed and then extracted twice at pH 10 with butyl chloride. Oxycodone and codeine (internal standard) were eluted with a mixture of methanol, acetonitrile, and 0.01 pH 7 phosphate buffer to which 40 mg/1 of cetyltrimethylammonium bromide (cetavlon) was added at ambient temperature and detected with electrochemical detection. The addition of cetavlon to the mobile phase markedly reduced the interaction between oxycodone and Si-OH groups on the stationary phase of the HPLC column, so that the organic content of the mobile phase could be reduced from 80 to 25%. Quantitation was achieved using the peak height ratio of oxycodone to codeine. The assay is currently being used for the study of oxycodone pharmacokinetics after single oral doses of 10 and 20 mg and single rectal doses of 30 mg.
Keyword Cetyltrimethylammonium bromide
Electrochemical detection
Liquid chromatography
Oxycodone
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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