Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus

Manners J.M., Davidson A.D. and Scott K.J. (1985) Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus. Plant Molecular Biology, 4 5: 275-283. doi:10.1007/BF02418246


Author Manners J.M.
Davidson A.D.
Scott K.J.
Title Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1985
Sub-type Article (original research)
DOI 10.1007/BF02418246
Volume 4
Issue 5
Start page 275
End page 283
Total pages 9
Publisher Martinus Nijhoff/Dr. W. Junk Publishers
Subject 1303 Specialist Studies in Education
1300 Biochemistry, Genetics and Molecular Biology
1311 Genetics
1110 Nursing
1102 Cardiovascular Medicine and Haematology
Abstract Pairs of susceptible and resistant, near-isogenic cultivars of Hordeum vulgare which differ for the Mla, Mlk and Mlp genes for resistance to Erysiphe graminis f. sp. hordei were inoculated with race 3 of this pathogen and patterns of protein synthesis associated with primary infection mapped using pulse-labelling with L-[35S]methionine and 2-dimensional electrophoresis. Extraction of proteins with buffer containing detergent revealed the enhanced synthesis of 5 and 8 polypeptides at 25 and 30 h respectively after inoculation of barley carrying the Mla gene (cvMla). The enhanced synthesis of these same polypeptides together with 11 additional polypeptides was observed at 48 h and 72 h after inoculation of barley carrying either the Mlp (cvMlp) or Mlk (cvMlk) genes. The labelling of several major constitutive polypeptides was suppressed in cvMla at 24 h after inoculation; the labelling of six of these polypeptides was also suppressed in both cvMlp and cvMlk but not until 48 and 72 h after inoculation. These results indicate that changes occur in the synthesis of some common polypeptides following infection of cultivars carrying different resistance genes but the timing and extent of these changes varies with the resistance gene in the host.
Keyword 2-dimensional electrophoresis
Erysiphe graminis
Hordeum vulgare
protein synthesis
resistance genes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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