A semiautomated method for the estimation of ionic calcium activity and concentration in biological solutions

Cham B.E. (1972) A semiautomated method for the estimation of ionic calcium activity and concentration in biological solutions. Clinica Chimica Acta, 37 C: 5-14. doi:10.1016/0009-8981(72)90409-3


Author Cham B.E.
Title A semiautomated method for the estimation of ionic calcium activity and concentration in biological solutions
Journal name Clinica Chimica Acta   Check publisher's open access policy
ISSN 0009-8981
Publication date 1972
Sub-type Article (original research)
DOI 10.1016/0009-8981(72)90409-3
Volume 37
Issue C
Start page 5
End page 14
Total pages 10
Subject 1303 Specialist Studies in Education
1308 Clinical Biochemistry
Abstract A semiautomated method for the determination of ionic calcium in plasma and urine with little preliminary sample treatment is reported. Separation of ionic calcium from interfering materials is achieved by dialysis under "physiological" conditions for plasma and absorption on Norit for urines. The activity of ionic calcium is determined colorimetrically using a metal complexing dye (tetramethylmurexide) which is not pH dependent. Only sodium interfered with the readings but this interference was easily removed. In 18 normal subjects the plasma ionized calcium concentration was 4.00 ± 0.40 mg%. The precision was a relative S.D. of 0.20%. The urinary ionic calcium concentration range of eight normal subjects was 42.2 to 146.1 mg/24 h with a relative S.D. of 33.84%. The proportion of plasma ionized and bound calcium as a percentage of total calcium was 41.17 ± 2.06% and 58.83 ± 2.06%, respectively. For urine these values were 50.01 ± 6.67% and 49.93 ± 6.67%. Reproducibility and recovery data are presented. The calculation of the dissociation constant of calcium tetramethylmurexide complex is also shown.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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