A method for analysing the clonal precursors of concanavalin A-induced suppressor cells

Good M.F., Halliday J.W. and Powell L.W. (1985) A method for analysing the clonal precursors of concanavalin A-induced suppressor cells. Journal of Immunological Methods, 80 2: 163-175. doi:10.1016/0022-1759(85)90018-3


Author Good M.F.
Halliday J.W.
Powell L.W.
Title A method for analysing the clonal precursors of concanavalin A-induced suppressor cells
Journal name Journal of Immunological Methods   Check publisher's open access policy
ISSN 0022-1759
Publication date 1985-06-25
Sub-type Article (original research)
DOI 10.1016/0022-1759(85)90018-3
Volume 80
Issue 2
Start page 163
End page 175
Total pages 13
Subject 1305 Biotechnology
2403 Immunology
Abstract Spleen cells from 3 different strains of mice (C57 (H-2b), CBA (H-2k) and BALB/c (H-2d)) were stimulated in vitro with different concentrations of concanavalin A (CA) for 48 h. This resulted in the production of cells capable of inhibiting the generation of alloantigen-specific cytotoxic T lymphocytes (CTL) in a mixed lymphocyte culture (MLC). 1 μg/ml was an effective concentration of CA to induce C57 and BALB/c suppressor cells (SC), but 5 μg/ml CA was required to induce CBA SC. SC precursors (SC-P) were shown to be radiosensitive and the results suggest that SC themselves may be radiosensitive. SC were effective in the presence of added interleukin-2 (IL-2). SC were then induced at limit dilution in microwells in a volume of 25 μl. A MLC (200 μl) was then (after 48 h) added to each microwell. This resulted in a dilution of the concentration of CA to a level below which it was effective at inducing suppression. Cytotoxicity was then assessed 7 days later. It was thus possible to analyse SC-P at the clonal level and estimate their frequency. The frequency of C57 splenic SC-P (active against a C57 anti-BALB/c MLC) was 14.4 × 10-6, the frequency of CBA splenic SC-P (active against a CBA anti-BALB/c MLC) was 92.3 × 10-6, and the frequency of BALB/c splenic SC-P (active against a BALB/c anti-CBA MLC) was 15.8 × 10-6. It was possible to analyse SC-P at a clonal level whether or not the MLC contained added IL-2. SC and SC-P were shown to be sensitive to anti-Thy-1 and complement.
Keyword clonal
concanavalin A
limit dilution
suppressor cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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