Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila

Gobius, Kari S. and Pemberton, John M. (1988) Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila. Journal of Bacteriology, 170 3: 1325-1332.

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Author Gobius, Kari S.
Pemberton, John M.
Title Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila
Formatted title
Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
1098-5530
Publication date 1988-03
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 170
Issue 3
Start page 1325
End page 1332
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The structural gene for excreted amylase from Aeromonas hydrophila JMP636 has been cloned within a 2.1-kilobase SmaI fragment of DNA. The amylase gene is transcribed from its own promoter in Escherichia coli, producing a gene product of M49,000. The amylase gene product is secreted to the periplasm of E. coli; however, it is not excreted. Nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. A potential signal peptide of 21 amino acid residues is present at the NH2 terminal of the predicted protein. Three regions of homology with other procaryotic and eucaryotic α-amylases were detected within the predicted amino acid sequence.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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