Lipid and protein histochemistry of enamel - Effects of fluoride

Patterson C.M., Kruger B.J. and Daley T.J. (1977) Lipid and protein histochemistry of enamel - Effects of fluoride. Calcified Tissue Research, 24 1: 119-125. doi:10.1007/BF02223305


Author Patterson C.M.
Kruger B.J.
Daley T.J.
Title Lipid and protein histochemistry of enamel - Effects of fluoride
Journal name Calcified Tissue Research   Check publisher's open access policy
ISSN 0008-0594
Publication date 1977
Sub-type Article (original research)
DOI 10.1007/BF02223305
Volume 24
Issue 1
Start page 119
End page 125
Total pages 7
Publisher Springer-Verlag
Subject 1310 Endocrinology
Abstract Staining reactions for a number of histochemical procedures for lipophilic staining and protein were studied in the enamel matrix along the length of rat incisors. Sudan Black gave a positive stain across the whole thickness of very early enamel (up to 30 μm) but this staining only continued as a narrow band close to the ameloblasts as the enamel matured. A variety of tests for protein produced almost identical staining patterns in enamel matrix up to 100 μm thick. Since the pattern of "lipid" staining persisted, after using a number of procedures which could normally be expected to remove lipid, it is suggested that Sudan Black positive staining may be due to lipophilic protein rather than lipid itself. Fluoride did not significantly alter the staining reactions for "lipid" and protein but did proceduce matrix which was much more effectively stained by cross-linking agents FFDNB and FF sulphene.
Keyword Enamel
Histochemistry
Lipid
Protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 14 Jun 2016, 01:04:51 EST by System User